Skip to main content
. 2023 Aug 26;14(8):566. doi: 10.1038/s41419-023-06091-6

Fig. 7. The function of USP14 in LPO and inflammation depends on CYP2E1.

Fig. 7

A Analysis of cellular TG levels in AML12 cells under CMZ treatment and corresponding plasmid transfection. B MitoSOX staining of AML12 cells under CMZ treatment and corresponding plasmid transfection (n = 3). Scale bar, 100 μm. C Expression of inflammation-related genes in AML12 cells under CMZ treatment and corresponding plasmid transfection (n = 4). D–F Effect of CMZ and Ad-USP14 adenovirus injections in mice on LW/BW ratios, liver TG and TC levels, serum AST and ALT levels. G GTT and ITT results for each group of mice (n = 12). *HFD-Ad-Con vs. HFD-Ad-USP14; #HFD-Ad-Con vs. HFD-Ad-Con+CMZ. *,#P < 0.05; **,##P < 0.01. There was no difference between the other groups. H–J Representative images of H&E staining, Oil Red O staining, and PSR staining of the livers in each group and the corresponding NAS scores and PSR areas. Scale bar, 50 μm. Lowercase letters indicate post hoc analyses significance (P < 0.05) and groups with different letters are statistically different per post hoc comparison. NS not significant.