Skip to main content
. Author manuscript; available in PMC: 2024 Sep 1.
Published in final edited form as: Dev Biol. 2023 Jun 3;501:20–27. doi: 10.1016/j.ydbio.2023.05.003

Figure 1. mCherry-Rap1a and Afadin localization during stages of lumen formation.

Figure 1.

A. Immunofluorescence of two-cell stage MDCK spheroids with Afadin (green), mCherry-Rap1a (red), and podxl (blue) prior to lumen formation.

B. Immunofluorescence as in A at the apical membrane initiation site (AMIS) stage.

C. Immunofluorescence at the lumen stage.

Arrows depict Afadin at lateral membranes and arrowheads show Afadin at apical-lateral junctions. Results are representative of at least 2 independent experiments. Scale bars: 5 μm.