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. Author manuscript; available in PMC: 2024 Sep 1.
Published in final edited form as: Dev Biol. 2023 Jun 3;501:20–27. doi: 10.1016/j.ydbio.2023.05.003

Figure 5. Absence of Rap1 leads to mislocalization of Afadin in vivo in nephron and ureteric bud epithelia.

Figure 5.

A. E14.5 control (Rap1af/f; Rap1bf/f) and mutant (Rap1af/f; Rap1bf/f; Six2-cre) kidneys lacking Rap1a/b from metanephric mesenchyme were immunostained with Afadin (white), NCAM (green) and Muc1 (magenta). Scale: 10 μm.

B. E14.5 control (Rap1af/f; Rap1bf/f) and mutant (Rap1af/f; Rap1bf/f; HoxB7-cre) kidneys lacking Rap1a/b from metanephric mesenchyme were immunostained with Afadin (white), E-cadherin (green) and Muc1 (magenta). Single images and projected z-stacks (6 μm) are indicated as 2D and 3D, respectively. Scale: 10 μm.

Results are representative of n = 3 for each genotype.