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SARS-CoV-2-neutralizing Ab in human milk is maintained for at least 9 months after infection. A proxy neutralization assay that measures Abs specific for the spike RBD, which block the RBD-ACE2 interaction, was performed using a premade kit according to instructions (GenScript). Dotted line: background neutralization level of COVID-19-naïve milk. Paired t-test was used for the analysis. PI, postinfection; RBD, receptor-binding domain.
