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Effect of inoculum and pH on the biofilm formation. Logarithmic-phase cells of Salmonella enteritidis 5996 were resuspended in ATM to an OD590 of 0.125 to 1.4. The cells were incubated as described in Materials and Methods, and the pH was measured and biofilm formation was monitored visually at 2 h of incubation. Biofilm formation was subjectively quantified from + to +++.
