Figure 7.
The combination of CD44 siRNA and 5-FU reduced stemness properties in HT-29 cells. (A) Spheroid formation assay was performed. Data indicated that single groups had a larger size of spheroids than the combination CD44 siRNA/5-FU group. (B) The colony formation assay demonstrated the smaller size of formed colonies in the combination group compared to the control and separate treatments. (C) mRNA expression levels of Nanog and Sox2 after transfection of CD44 siRNA with or without 5-FU were evaluated via qRT‐PCR. Data represent the means ± SD of experiments. *P < 0.05; ***P < 0.001; ****P< 0.0001