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. 2023 Jun 17;10(24):2301505. doi: 10.1002/advs.202301505

Figure 1.

Figure 1

Genetic deletion of Bmal1 in mice promotes tumor growth and fibrosis. A) Representative picture of subcutaneous mouse CRC tumors grown in Bmal1+/+ and Bmal1−/− mice. B) Quantification of CRC tumor growth rates, tumor weight, and tumor relative mass increase in Bmal1+/+ and Bmal1−/− mice (n = 7 mice per group). C) Representative picture of subcutaneous PDAC tumors grown in Bmal1+/+ and Bmal1−/− mice. D) Quantification of PDAC tumor growth rates, tumor weights, and tumor relative mass increase in Bmal1+/+ and Bmal1−/− mice (n = 6 mice per group). E) H&E and Masson's trichrome staining of CRC tumors (n = 6 random fields per group). F) Representative micrographs and quantification of immunofluorescence staining of CRC tumor tissues in Bmal1+/+ and Bmal1−/− mice with fibroblast markers: FAP (green); α‐SMA (green) plus CD31 (red); DESMIN (red) plus ENDOMUCIN (green); PDGFRα (green); and PDGFRβ (green) (n = 6 random fields per group). G) H&E and Masson's trichrome staining of PDAC tumors in Bmal1+/+ and Bmal1−/− mice (n = 6 random fields per group). H) Representative micrographs and quantification of immunofluorescence staining of PDAC tumor tissues with fibroblast markers: FAP (green); α‐SMA (green) plus CD31 (red); DESMIN (red) plus ENDOMUCIN (green); PDGFRα (green); and PDGFRβ (green) (n = 6 random fields per group). I) Representative pictures of orthotopic HCC tumors grown in Bmal1+/+ and Bmal1−/− mice. J) Quantification of orthotopic HCC tumor, liver weights, and relative liver mass increase in Bmal1+/+ and Bmal1−/− mice (n = 3 mice per group). K) H&E and Masson's trichrome staining of orthotopic HCC tumors in Bmal1+/+ and Bmal1−/− mice (n = 6 random fields per group). L) Representative micrographs and quantification of immunofluorescence staining of HCC tumor tissues with fibroblast markers: FAP (green); α‐SMA (green) plus CD31 (red); DESMIN (red) plus ENDOMUCIN (green); PDGFRα (green); and PDGFRβ (green) (n = 6 random fields per group). Data presented as mean ± S.E.M. *P < 0.05; **P < 0.01; ***P < 0.001; two‐tailed student t‐test. Scale bar = 50 µm.