Figure 3.

Identification of cell types in D85 hypothalamus organoids by scRNA-seq

(A) Schematic overview of the scRNA-seq approach from sorting to sequencing and analysis. scRNA-seq, single-cell RNA sequencing.

(B) Left: UMAP plot of the broad cell type categories in the dataset derived by supervised clustering and classification. UMAP, Uniform Manifold Approximation and Projection. Right: Pie chart showing distribution of the different broad cell type populations.

(C) Dot plot showing average gene expression and proportion of cells expressing representative markers for each broad cell type.

(D) Left: UMAP plot of the neuronal subtype categories within the neurons of the broad cell types derived by unsupervised clustering. Right: Pie chart showing distribution of the different neuronal subtype populations.

(E) Dot plot showing average gene expression and proportion of cells expressing representative markers for each neuronal subtype.

See also Figures S2–S8 and Tables S1 and S2.