Figure 5.

Molecular diversity within the DN subtypes in hypothalamus organoids

(A) UMAP plots illustrating the distribution of annotated DN subtypes.

(B) Dot plot showing the expression of select markers that were used to annotate the DN subtypes.

(C) RNA velocity arrows overlaid on the DN subtype UMAP, showing pseudotime trajectories.

(D) RNA velocity heatmap showing the distribution of the DN subtype markers across the pseudotime trajectories.

(E) IPA results showing the pathways that were upregulated (yellow = increased) or downregulated (blue = decreased) in DNs compared to non-DN neurons in the organoids based on significance or p value. IPA, Ingenuity Pathway Analysis.

See also Figure S10 and Table S3.