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. 2023 Aug 14;14:1172862. doi: 10.3389/fmicb.2023.1172862

Table 1.

Permanova (Permutational Multivariate Analysis of Variance Using Distance Matrices) and Betadisper (Multivariate Homogeneity of Groups Dispersions) models on the subsetted fungal and bacterial datasets according to slice and treatment (leaf-T1, leaf-T4, near soil-T1, T1-control, near soil-T4, far soil-T1, far soil-T4, and T4-control).

Fungi Permanova Betadisper
Group Factor Df Sum.Sq F R 2 P.adj Sum.Sq F P.adj
T1-Leaf Plant 2 4.22612 14.90845 0.29459 0.00080 0.0132 0.5592 0.5743
T1-Leaf Pore 1 0.44086 3.11042 0.03073 0.01840 0.0883 13.6263 0.0004
T1-Nearsoil Plant 2 2.85516 11.13978 0.24329 0.00080 0.0233 1.7466 0.1824
T1-Nearsoil Pore 1 0.44797 3.49560 0.03817 0.00880 0.0425 14.7884 0.0003
T1-Farsoil Pore 1 0.74554 4.85338 0.07261 0.00080 0.3237 71.3412 0.0000
T1-Farsoil Moisture 1 0.42277 2.75218 0.04118 0.00800 0.0063 0.5691 0.4537
T1-Control Pore 1 0.32534 2.38360 0.10468 0.00040 0.0427 10.4906 0.0041
T4-Leaf Plant 2 4.03977 15.91940 0.28679 0.00080 0.0507 2.0021 0.1436
T4-Leaf Pore 1 0.92249 7.27047 0.06549 0.00080 0.0299 4.4552 0.0387
T4-Leaf Time 2 0.81393 3.20743 0.05778 0.00080 0.0202 1.2462 0.2946
T4-Leaf Moisture 1 0.55793 4.39724 0.03961 0.00160 0.0067 0.8304 0.3656
T4-Leaf Pore:Moisture 1 0.37736 2.97408 0.02679 0.02000 - - -
T4-Nearsoil Plant 2 1.60929 6.19018 0.15103 0.00080 0.0094 0.5129 0.6012
T4-Nearsoil Pore 1 0.40427 3.11009 0.03794 0.00160 0.2103 36.0006 0.0000
T4-Nearsoil Moisture 1 0.34383 2.64510 0.03227 0.01120 0.0066 0.7250 0.3976
T4-Farsoil Pore 1 0.35941 2.63748 0.04163 0.01440 0.3542 56.6875 0.0000
T4-Farsoil Plant:Pore:Moisture 1 0.40467 2.96966 0.04687 0.00480 - - -
T4-Control Pore 1 0.30512 2.49281 0.10162 0.01240 0.1199 18.9037 0.0003
Bacteria
Group Factor Df Sum.Sq F R 2 P.adj Sum.Sq F P.adj
T1-Leaf Plant 2 7.99222 33.13792 0.39641 0.00080 0.2459 32.4638 0.0000
T1-Leaf Moisture 1 1.69730 14.07491 0.08418 0.00080 0.0029 0.4915 0.4857
T1-Leaf Time 2 1.22462 5.07759 0.06074 0.00080 0.0127 1.4587 0.2398
T1-Leaf Pore 1 1.01912 8.45110 0.05055 0.00080 0.0002 0.0480 0.8273
T1-Nearsoil Plant 2 0.96853 5.43286 0.12532 0.00080 0.0010 0.6545 0.5231
T1-Nearsoil Time 2 0.53755 3.01534 0.06956 0.00080 0.0003 0.1776 0.8377
T1-Nearsoil Pore 1 0.45416 5.09507 0.05877 0.00080 0.0018 2.0833 0.1536
T1-Nearsoil Moisture 1 0.25621 2.87432 0.03315 0.00080 0.0012 1.7753 0.1873
T1-Farsoil Time 2 0.52353 2.90084 0.08460 0.00080 0.0000 0.0285 0.9719
T1-Farsoil Plant 2 0.40441 2.24080 0.06535 0.00080 0.0013 0.9940 0.3765
T1-Farsoil Pore 1 0.36626 4.05886 0.05919 0.00080 0.0032 5.2212 0.0261
T1-Control Time 2 0.36715 2.03374 0.16551 0.00040 0.0220 2.1863 0.1398
T1-Control Pore 1 0.19382 2.14727 0.08737 0.00960 0.0427 10.4906 0.0041
T4-Leaf Plant 2 7.65287 37.25758 0.40881 0.00080 0.2337 20.6704 0.0000
T4-Leaf Pore 1 1.67765 16.33514 0.08962 0.00080 0.0187 3.0142 0.0873
T4-Leaf Time 2 1.57711 7.67806 0.08425 0.00080 0.0400 5.0084 0.0095
T4-Leaf Moisture 1 0.98904 9.63021 0.05283 0.00080 0.0010 0.3096 0.5799
T4-Leaf Pore:Moisture 1 0.52480 5.10997 0.02803 0.00720 - - -
T4-Nearsoil Plant 2 0.88452 5.07130 0.12185 0.00080 0.0012 0.7689 0.4678
T4-Nearsoil Pore 1 0.39821 4.56619 0.05486 0.00080 0.0010 1.1113 0.2957
T4-Nearsoil Time 2 0.32738 1.87699 0.04510 0.00320 0.0003 0.2050 0.8152
T4-Nearsoil Moisture 1 0.25161 2.88516 0.03466 0.00080 0.0002 0.2241 0.6375
T4-Nearsoil Pore:Moisture 1 0.19160 2.19708 0.02639 0.00880 - - -
T4-Farsoil Time 2 0.34319 1.94229 0.05863 0.00080 0.0013 0.8474 0.4338
T4-Farsoil Pore 1 0.30714 3.47648 0.05247 0.00080 0.0010 1.1832 0.2812
T4-Farsoil Plant 2 0.29821 1.68774 0.05094 0.00080 0.0003 0.1571 0.8550
T4-Farsoil Moisture 1 0.15576 1.76304 0.02661 0.00960 0.0000 0.0122 0.9124
T4-Farsoil Plant:Pore:Moisture 1 0.14815 1.67689 0.02531 0.01440 - - -
T4-Control Time 2 0.25182 1.49254 0.11267 0.00240 0.1105 4.4663 0.0242
T4-Control Pore 1 0.19786 2.34541 0.08853 0.00040 0.1199 18.9037 0.0003
T4-Control Moisture 1 0.16089 1.90716 0.07199 0.00080 0.0039 0.3283 0.5724

Time was treated as a fixed effect and included as a factor variable in the models (Time + Plant * Pore * Moisture, and Time + Pore * Moisture for just the control samples). The order of the factors was chosen to first remove variance from variables with the highest impact in the models and better investigate the remaining variables. Only factors with significant (P ≤ 0.05) Benjamini-Hochberg-corrected P-values are displayed. Factor explaining the highest R2 for each model and significant adjusted p-values are reported in bold.