TABLE 1.

Culture of ovine and bovine clinical samples known to contain M. paratuberculosis by using radiometric medium, HEYM, and identification of M. paratuberculosis by IS900 PCR with a sample from the primary culture^a

Animal no.	Species	Sample	Time to positive PCR result (wk)	GI when the culture PCR was positive	Result of culture on HEYM
1	Ovine	Ileum	1	236	Negative
2	Ovine	Lymph node	2	10	Negative
3	Ovine	Lymph node	2	421	Negative
4	Ovine	Ileum	1	999	Negative
5	Ovine	Lymph node	4b	999	Negative
6	Ovine	Feces	2	512	Negative
7	Ovine	Feces	2	664	Negative
8	Ovine	Feces	4c	999	Negative
9	Bovine	Feces	5c	913	Positive, 10 wk
10	Bovine	Feces	4d	20	Positive, 10 wk
11	Bovine	Feces	6c	566	Positive, 10 wk
12	Bovine	Feces	3	23	Positive, 10 wk
Control	M. avium	Laboratory culture	—e	999	Not tested
Control	M. paratuberculosis	Laboratory culture	1	26	Not tested

^aRadiometric cultures were incubated for 8 weeks, and HEYM cultures were incubated for 20 weeks. 

^bSamples from culture were PCR negative for the first 2 weeks that they were tested but were PCR positive in all later weeks. 

^cA sample from culture was PCR negative when the first sample was obtained but PCR positive in all later weeks. 

^dA sample from culture was PCR negative in week 5. 

^e—, did not test positive by IS900 PCR.