TABLE 2.

Culture of caprine and ovine clinical samples with radiometric medium and identification of M. paratuberculosis^a by IS900 PCR

Animal no.	Species	Sample	Status of animal based on histology and Ziehl-Neelsen smear of feces or tissuesb	Time to positive PCR result (wk)	GI when the culture was PCR positive
1	Goat	Lymph node	I	2	411
		Feces	I	3	269
2	Goat	Lymph node	U	4	438
		Feces	U	5	28
3	Goat	Lymph node	I	2	480
		Feces	I	5c	999
4	Goat	Lymph node	U	−d	−
		Feces	U	5	10
5	Goat	Lymph node	U	−	−
		Feces	U	−	−
6	Sheep	Lymph node	I	4	35
		Feces	I	−	−
7	Sheep	Lymph node	U	−	−
		Feces	U	−	−
8	Sheep	Lymph node	U	−	−
		Feces	U	−	−
9	Sheep	Lymph node	U	4	190
		Feces	U	5	194
10	Sheep	Lymph node	U	−	−
		Feces	U	−	−
11	Sheep	Lymph node	U	−	−
		Feces	U	−	−
12	Sheep	Lymph node	I	5c	586
		Feces	I	−	−
13	Sheep	Lymph node	U	−	−
		Feces	U	−	−
14	Sheep	Lymph node	I	2e	331
		Feces	I	5c	999
15	Sheep	Lymph node	U	−	−
		Feces	U	−	−
16	Sheep	Lymph node	U	−	−
		Feces	U	−	−

^aThe animals were from a farm where livestock were known to have been infected with M. paratuberculosis and were selected on the basis of serological results. Radiometric cultures were incubated for 8 weeks. The results of culture on HEYM were negative at 20 weeks in each case. 

^bI, infected; U, uninfected. 

^cSamples from culture were PCR negative when they were first tested but were PCR positive in later weeks. 

^d−, negative. 

^eThe sample from culture was PCR negative in week 4.