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. 1983 Feb;59(1):41–43. doi: 10.1136/sti.59.1.41

Plasmid profile, serogrouping, and auxotyping of Neisseria gonorrhoeae isolates from Africa.

T O Odugbemi, S T Brown, J Biddle, S Johnson, G Perkins, W DeWitt, W L Albritton
PMCID: PMC1046128  PMID: 6402263

Abstract

The plasmid patterns of 90 isolates of Neisseria gonorrhoeae (including 39 penicillinase-producing strains) originating from various countries in Africa were determined. Serogrouping by coagglutination and auxotyping were used to characterise the isolates. The 4.4-megadalton plasmid was present in seven isolates out of 39 penicillinase-producing strains, two of which occurred with a conjugative 24.5-megadalton plasmid. The African strains were predominantly serogroup WI and wild type. Arginine-dependent isolates were as common as proline-dependent types.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson B., Odugbemi T., Johnson S. Penicillinase producing Neisseria Gonorrhoeae strains from Nigeria with Far Eastern type plasmid. Lancet. 1982 Mar 20;1(8273):676–676. [PubMed] [Google Scholar]
  2. Ansink-Schiper M. C., van Embden J. D., van Klingeren B., Woudstra R. Further spread of plasmids among different auxotypes of penicillinase-producing gonococci. Lancet. 1982 Feb 20;1(8269):445–445. doi: 10.1016/s0140-6736(82)91640-3. [DOI] [PubMed] [Google Scholar]
  3. Bygdeman S., Kallings I., Danielsson D. Serogrouping and auxotyping for epidemiological study of beta-lactamase-producing Neisseria gonorrhoeae strains isolated in Sweden. Acta Derm Venereol. 1981;61(4):329–334. [PubMed] [Google Scholar]
  4. Dillon J. R., Pauzé M. Appearance in Canada of Neisseria gonorrhoeae strains with a 3.2 megadalton penicillinase-producing plasmid and a 24.5 megadalton transfer plasmid. Lancet. 1981 Sep 26;2(8248):700–700. doi: 10.1016/s0140-6736(81)91037-0. [DOI] [PubMed] [Google Scholar]
  5. Handsfield H. H., Sandström E. G., Knapp J. S., Perine P. L., Whittington W. L., Sayers D. E., Holmes K. K. Epidemiology of penicillinase-producing Neisseria gonorrhoeae infections: analysis by auxotyping and serogrouping. N Engl J Med. 1982 Apr 22;306(16):950–954. doi: 10.1056/NEJM198204223061602. [DOI] [PubMed] [Google Scholar]
  6. Jephcott A. E., Dickgiesser N., McClean A. N. Penicillinase-producing gonococci in Britain. Lancet. 1981 Aug 1;2(8240):247–248. doi: 10.1016/s0140-6736(81)90487-6. [DOI] [PubMed] [Google Scholar]
  7. Johnston N. A., Kolator B. Emergence in Britain of beta-lactamase-producing gonococci with new plasmid combination. Lancet. 1982 Feb 20;1(8269):445–446. doi: 10.1016/s0140-6736(82)91641-5. [DOI] [PubMed] [Google Scholar]
  8. Knapp J. S., Holmes K. K. Disseminated gonococcal infections caused by Neisseria gonorrhoeae with unique nutritional requirements. J Infect Dis. 1975 Aug;132(2):204–208. doi: 10.1093/infdis/132.2.204. [DOI] [PubMed] [Google Scholar]
  9. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. doi: 10.1128/aac.1.4.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Odugbemi T. O., Hafiz S., McEntegart M. G. Penicillinase-producing Neisseria gonorrhoeae: detection by starch paper technique. Br Med J. 1977 Aug 20;2(6085):500–500. doi: 10.1136/bmj.2.6085.500. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Perine P. L., Thornsberry C., Schalla W., Biddle J., Siegel M. S., Wong K. H., Thompson S. E. Evidence for two distinct types of penicillinase-producing Neisseria gonorrhoeae. Lancet. 1977 Nov 12;2(8046):993–995. doi: 10.1016/s0140-6736(77)92891-4. [DOI] [PubMed] [Google Scholar]
  13. Roberts M., Falkow S. Conjugal transfer of R plasmids in Neisseria gonorrhoeae. Nature. 1977 Apr 14;266(5603):630–631. doi: 10.1038/266630a0. [DOI] [PubMed] [Google Scholar]
  14. Sandstrom E. G., Knapp J. S., Buchanan T. B. Serology of Neisseria gonorrhoeae: W-antigen serogrouping by coagglutination and protein I serotyping by enzyme-linked immunosorbent assay both detect protein I antigens. Infect Immun. 1982 Jan;35(1):229–239. doi: 10.1128/iai.35.1.229-239.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sandström E., Danielsson D. Serology of Neisseria gonorrhoeae. Classification by co-agglutination. Acta Pathol Microbiol Scand B. 1980 Feb;88(1):27–38. doi: 10.1111/j.1699-0463.1980.tb02600.x. [DOI] [PubMed] [Google Scholar]

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