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. 2021 Nov 26;104(4):00368504211058036. doi: 10.1177/00368504211058036

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — The effect of NLRX1 on MAVS-RLR-mediated downstream signaling pathways in HBV serum-treated HepG2-NTCP cells. The HepG2-NTCP cells were transfected with NLRX1-overexpressed plasmid or NLRX1 siRNA for 48 h, and then were treated with HBV serum for another 48 h prior further analysis. (A) Western blotting was used to analyze the protein level of p65, p-p65, IRF3, IRF7, p-IRF3, and p-IRF7. (B) The phosphorylation ratios of p65, IRF3, and IRF7 were analyzed on the basis of the gray value. (C) The effect of NLRX1 on the activity of IFN-α, IFN-β, and IL-6 promoters was calculated using a luciferase reporter assay. All experiments were independently repeated for three times. One-way ANOVA was applied for the comparison of three sets of data.

HBV: hepatitis B virus; ANOVA: analysis of variance.

*: P < 0.05; **: P < 0.01.