Figure 3.

miR-200a supplementation attenuated colonic inflammation accumulation and suppressed colonic apoptosis in DSS-treated mice. (A–D) Colonic inflammatory factors (IL-1β, TNF-α, IL-6, IL-10) detected using ELISA kits. (E, F) Representative images of TUNEL staining in colon samples from different experimental groups. (Original magnifications, ×200, scale bars =50 μm; ×400, scale bars =20 μm). The images of TUNEL positive for quantification were analyzed using Image J software. (G) Representative immunoblot bands for the Bax and Bcl-2 proteins in the colon of mice. GAPDH was used as a loading control. (H, I) Histogram of relative expression of Bax and Bcl-2 proteins in the colon of mice. Data are provided as mean ± SEM and reflect six experiments, analyzed using one-way ANOVA with Tukey post-hoc analysis. ^∗P < 0.05; ns, not significant.