TABLE 2.
Sampleb | Method | Mean | SD | CV (%) |
---|---|---|---|---|
1 | bDNA | 297,066 | 72,205 | 24.3 |
RT-PCR | 780,826 | 267,937 | 34.3 | |
2 | bDNA | 24,011 | 10,571 | 44.0 |
RT-PCR | 110,843 | 47,322 | 42.7 |
Each sample was tested in triplicate in six separate runs for a total of 18 replicates.
Samples 1 and 2 were 1.5 × 106- and 1.5 × 107-fold dilutions, respectively, of an HIV-1 SF2 stock suspension quantitated at 2.48 × 109 virus particles/ml.