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. 1998 Mar;36(3):716–720. doi: 10.1128/jcm.36.3.716-720.1998

TABLE 2.

Between-run reproducibility of bDNA and RT-PCR for quantitation of HIV-1 RNA copies per milliliter at two concentrationsa

Sampleb Method Mean SD CV (%)
1 bDNA 297,066 72,205 24.3
RT-PCR 780,826 267,937 34.3
2 bDNA 24,011 10,571 44.0
RT-PCR 110,843 47,322 42.7
a

Each sample was tested in triplicate in six separate runs for a total of 18 replicates. 

b

Samples 1 and 2 were 1.5 × 106- and 1.5 × 107-fold dilutions, respectively, of an HIV-1 SF2 stock suspension quantitated at 2.48 × 109 virus particles/ml.