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Cell viability in cartilage explants (n = 3). (a) Live/Dead staining of the control, overloaded, IL-1β challenged, and overloaded plus IL-1β challenged cartilage (green: live; red: dead). (b) Quantification of Live/Dead staining images. Overloading significantly reduced cell viability in the top zone of cartilage explants compared to the control or IL-1β group and to their respective middle zones (*vs ctrl, $vs IL-1β, #between top and middle zones, p < 0.05).
