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. Author manuscript; available in PMC: 2023 Aug 28.
Published in final edited form as: Cell Rep. 2021 Oct 19;37(3):109846. doi: 10.1016/j.celrep.2021.109846

Figure 1. Development of TOMM20-mNeonGreen tagged mouse model to confirm Rhod-2-AM loading into mitochondria.

Figure 1.

Panel a shows fluorescence images of mNeonGreen-tagged TOMM20 in a widefield image of a WT mouse embryo (left) and a TOMM20- mNeonGreen expressing embryo (right). Panel b shows a widefield image of an embryonic mouse heart expressing mNeonGreen TOMM20. Panel c shows an excised torso section showing TOMM20-mNeonGreen expression in skeletal muscle. Scale bar represent 2 mm, 500 μm, and 2 mm for panels a-c respectively. Panel d shows Stimulated Emission Depletion (STED) microscopy image from a slice of an ex vivo mNeonGreen labelled TOMM20 (green) heart loaded with Rhod-2 (red). This image is a slice from a larger six slice z-stack that was taken over an axial range of 0.8 mm2 and deconvolved. The scale bar represents 5 μm. An enlarged image from the box region of interest is shown in panel e, and a line profile of intensities through the dotted line shown in panel f.