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. Author manuscript; available in PMC: 2023 Aug 28.
Published in final edited form as: Cell Rep. 2021 Oct 19;37(3):109846. doi: 10.1016/j.celrep.2021.109846

Figure 6. Analysis of chromophores during isoproterenol treatment.

Figure 6.

Panel a shows the time course of peak absorbance of cytochrome a605 (purple) during isoproterenol treatment and washout. Cytochrome changes were analyzed at timepoints labeled 1 (control), 2 (transient hypoxia during isoproterenol treatment), and 3 (return from hypoxia). Panels b and c show the chromophores fit using LabView showing the difference spectra between times 2 vs 1, and 3 vs 1, respectively. Panel d shows the average peak difference absorbance values from chromophores in wild-type (red) and MCU-KO (black) taken before isoproterenol treatment (time 1) and after transient hypoxia time (time 3). N=5 biological replicates for each genotype.