KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| Rhod-2 AM | Cayman Chemical | CAS: 145037-81-6 |
| (−)-Isoproterenol hydrochloride | Sigma | CAS: 5984-95-2 |
| Ru360 | Sigma | CAS: 557440 |
| Experimental models: Cell lines | ||
| Human: HEK293T | ATTC | https://www.atcc.org/products/all/crl-3216.aspx#generalinformation |
| Experimental models: Organisms/strains | ||
| Mouse: MCU-KO line | Pan, et al 2013 | N/A |
| Mouse: TOMM20-mNeonGreen | This paper | N/A |
| Mouse: B6D2F1/J | The Jackson Laboratory | Stock No. 100006 |
| Mouse: C57BL/6N | Taconic Farms | https://www.taconic.com/mouse-model/black-6-b6ntac |
| Recombinant DNA | ||
| Plasmid: mNeonGreen-TOMM20-N-10 | Allele Biotechnology | https://reagents.allelebiotech.com/mneongreen-1/ |
| Vector: pSF-CAG-Kan | Sigma-Aldrich | Cat# OGS505-5UG |
| Oligonucleotides | ||
| Primer: AAAGGAGGTACCCACCATGGTGGGTCGGAACAG (Forward) | Integrated DNA Technologies | N/A |
| Primer: ACAACTTCTAGACTTGTACAGCTC GTCCATGC (Reverse) | Integrated DNA Technologies | N/A |
| Critical commercial assays | ||
| Wizard Genomic DNA Extraction kit | Promega | Cat# A1120 |
| Software and algorithms | ||
| LabVIEW Spectroscopy Program | This paper | N/A |
| Huygens Professional | Scientific Volume Imaging | Version 19.1; https://svi.nl/Huygens-Professional |
| LabChart | ADInstruments | Version 8.0; https://www.adinstruments.com/products/labchart/versions-and-licenses |