Table 6.
Day 0: E6SFB differentiation medium composition
| Components | Vendor | Cat. no. | Stock concentration | Final concentration | Volume (30 mL) |
|---|---|---|---|---|---|
| Essential 6 medium | Gibco | A1516401 | - | 98% (v/v) | 29.4 mL |
| Matrigel, growth factor reduced | Corning | 354230 | - | 2% (v/v) | 600 μL |
| SB (TGF-β inhibitor) | Stemgent | 04-0010-05 | 10 mM | 10 μM | 30 μL |
| Recombinant human bFGF | PeproTech | 100-18B | 200 μg/mL | 4 ng/mL | 0.6 μL |
| Recombinant human BMP4 | PeproTech | 120-05 | 100 μg/mL | 5 ng/mL | 1.5 μL |
| Normocin | InvivoGen | Ant-nr-1 | 50 mg/mL | 100 μg/mL | 60 μL |