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. Author manuscript; available in PMC: 2023 Aug 28.
Published in final edited form as: Nat Protoc. 2022 Mar 23;17(5):1266–1305. doi: 10.1038/s41596-022-00681-y

Table 8.

OMM composition

Components Vendor Cat. no. Stock concentration Final concentration Volume (50 mL)
Advanced DMEM/F12 Gibco 12634010 - 49% (v/v) 24.5 mL
Neurobasal medium Gibco 21103049 - 49% (v/v) 24.5 mL
GlutaMAX supplement Gibco 35050061 100X 1X 500 μL
B-27 supplement, minus vitamin A Gibco 12587010 50X 0.5X 500 μL
N2 supplement Gibco 17502048 100X 0.5X 250 μL
2-Mercaptoethanol Gibco 21985023 55 mM 0.1 mM 91 μL
Normocin InvivoGen Ant-nr-1 50 mg/mL 100 μg/mL 100 μL