FIG. 3.

Southern blot hybridization of P. brasiliensis chromosome-sized DNA molecules with homologous probes. Chromosomal bands of isolates 113 and B-339 were separated on a Gene Navigator apparatus using the electrophoretic conditions described in Materials and Methods. Chromosomal DNA was transferred onto nylon filters and hybridized with the following radiolabeled probes: total genomic DNA of P. brasiliensis digested with AluI (A), a 600-bp fragment of the chitin synthase gene (GenBank accession no. YO9231) generated by PCR amplification of P. brasiliensis genomic DNA (B), and a 630-bp BamHI/HindIII fragment from the coding region of the gp43 antigen gene of P. brasiliensis (GenBank accession no. U26160) (C).