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. 2023 Aug 16;19(8):e1011563. doi: 10.1371/journal.ppat.1011563

Fig 3. Gain of function phenotype and cytolocalization of TvNlpC genes in T. vaginalis.

Fig 3

(A) Spotting mixed cultures of T. vaginalis and lactobacilli on MRS-agar plates reveals a greater reduction on bacterial numbers when the protozoan is expressing an unregulated, HA-tagged copy of TvNlpC_A1, TvNlpC_A2, TvNlpC_B3 or TvNlpC_B5 as compared to the empty vector. (B) Immunofluorescence microscopy and Western blot show that TvNlpC_B3 and _B5 are located in organelles. (Top panel) Immunofluorescence of T. vaginalis cells transfected with HA-tagged TvNlpC_B3 and _B5 and stained with FITC-conjugated antibody and DAPI. (Bottom panel) Cell fractionation of T. vaginalis transfected with HA-tagged TvNlpC_B3 and _B5 followed by Western blots, probed with antibodies against the HA-tagged protein (Anti-HA) or the organellar marker ferredoxin (Anti-Fd).