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. 2023 Aug 16;19(8):e1011591. doi: 10.1371/journal.ppat.1011591

Fig 3. Hepcidin-25 measurement in culture supernatants with HCV infection and in sera from hepatitis C patients.

Fig 3

(A) Hepcidin-25 concentrations in the supernatants of HCV-infected CREBH-KO cells and parental cells at 3 dpi at MOIs of 0, 0.2, and 1 were measured by the LC-MS/MS. As a positive control (PC), culture supernatants of cells transfected with the hepcidin-expressing plasmid were collected at 2 dpt and used. Expression of HCV Core and GAPDH in cells was assessed. (B) Changes in serum hepcidin-25 concentrations with decreasing viral loads in hepatitis C patients were analyzed. Paired serum samples from 26 hepatitis C patients were collected before (HCV positive) and after DAA therapy (HCV negative), and serum hepcidin-25 concentrations before vs. after viral elimination were compared. Significant differences between two groups before and after therapy were analyzed using Wilcoxon rank sum tests (***P<0.001).