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. Author manuscript; available in PMC: 2023 Aug 28.
Published in final edited form as: Methods Mol Biol. 2020;2124:141–159. doi: 10.1007/978-1-0716-0356-7_7

Table 2.

Summary of nanobiolistic transformation studies in plants

Species/tissue NP type Cargo Loading capacity (w/w) Delivery pressure (psi) Target distance (cm) Efficiency Reference
N. tabacum plantlets Au-capped MSNs (2.3 nm pores) GFP plasmid and inducer 0.1 DNA:MSN 650 10 35.6 ± 12.8 GFP+ foci per plantlet [34]
A. cepa epidermis Au-capped MSNs (10 nm pores) GFP or FITC-BSA protein, mCherry plasmid 0.625 BSA:MSN 0.15 GFP:MSN 0.01 DNA:MSN 1350 6 8% (GFP) and 28% (BSA) protein released [98]
Z. mays embryos Au-plated MSNs (10 nm pores) Cre protein 0.03 Cre:MSN 650 6 24 events out of 120 embryos [98]
O. sativa embryogenic calli AuNPs (50, 200, 600, 1000 nm) cry plasmid with hgh selectable marker Not reported 1100 5 5 events out of 12 putative lines [99]
A. cepa epidermis Au nanorods (NRs, 25 × 73 nm) mCherry plasmid 0.1 DNA: NRs 1350 4 77% of bombarded cells [97]