Table 2:
Primers used in the study
| Primers | Gene | Nucleotide sequence 5′- 3′ | Purpose |
|---|---|---|---|
| F1 | PNKP (human) | GCGTATGCGGaggTCAAACCCCG- | For introducing K142R mutation |
| R1 | PNKP (human) | TTCTTCGGCAGCTCAGCATC | For introducing K142R mutation |
| F2 | PNKP (human) | CGGGCGCGGGaggCTGCCAGCCG | For introducing K226R mutation |
| R2 | PNKP (human) | ATGCTCATCTGGTTGGTGAAGATCACCAGCTTGTAGCCCTCG | For introducing K226R mutation |
| F3 | PNKP (human) | GCGTATGCGGcagTCAAACCCCG | For introducing K142Q mutation |
| R3 | PNKP (human) | CGGGCGCGGGcagCTGCCAGCCG | For introducing K142Q mutation |
| F4 | PNKP (human) | CGGGCGCGGGcagCTGCCAGCCG | For introducing K226Q mutation |
| R4 | PNKP (human) | ATGCTCATCTGGTTGGTGAAGATCACCAGCTTGTAGCCC | For introducing K226Q mutation |
| F5 | HPRT (human) | TGGGATTACACGTGTGAACCAACC | LA-qPCR |
| R5 | HPRT (human) | GCTCTACCCTCTCCTCTACCGTCC | LA-qPCR |
| F6 | HPRT (human) | TGCTCGAGATGTGATGAAGG | SA-PCR |
| R6 | HPRT (human) | CTGCATTGTTTTGCCAGTGT | SA-PCR |
| F7 | POLB (human) | CATGTCACCACTGGACTCTGCAC | LA-qPCR |
| R7 | POLB (human) | CCTGGAGTAGGAACAAAAATTGCT | LA-qPCR |
| F8 | POLB (human) | AGTGGGCTGGATGTAACCTG | SA-PCR |
| R8 | POLB (human) | CCAGTAGATGTGCTGCCAGA | SA-PCR |
| F9 | RNAPOLII (human) | AGGGGGTGTGTGAAGCAAAA | LA-qPCR |
| R9 | RNAPOLII (human) | AGGGAGGCACATCTCTAGCA | LA-qPCR |
| F10 | RNAPOLII (human) | CGCATTGACTTGCGTTTCCA | SA-PCR/ChIP-qPCR |
| R10 | RNAPOLII (human) | CTGGGCAGCAACAGCCTTTA | SA-PCR/ChIP-qPCR |
| F11 | NANOG (human) | CTCCGGAATGGTAGTCTGAGAAGAA | LA-qPCR |
| R11 | NANOG (human) | ATTTAGGGCAGGCACAAGATGG | LA-qPCR |
| F12 | NANOG (human) | ACCTACCTACCCCAGCCTTT | SA-PCR |
| R12 | NANOG (human) | CATGCAGGACTGCAGAGATT | SA-PCR |
| F13 | OCT4 (human) | TCTGTGGCCTCACCCTATGA | LA-qPCR |
| R13 | OCT4 (human) | CAGACCTGTGGCAGGTATTGAA | LA-qPCR |
| F14 | OCT4 (human) | GAAGGTATTCAGCCAAACGA | SA-PCR |
| R14 | OCT4 (human) | GCACTGCAGGAACAAATTCT | SA-PCR |
| F15 | MyH2 (human) | AAAGCCTGCCAAGCCCTAAA | LA-qPCR |
| R15 | MyH2 (human) | TGGTCAGCATGGCAAGTGAA | LA-qPCR |
| F16 | MyH2 (human) | GGCTCAAACTGCTGAAGGAGA | SA-PCR |
| R16 | MyH2 (human) | TGGCACCAGGAGTTTTTGTCT | SA-PCR |
| F17 | POLB (mouse) | TATCTCTCTTCCTCTTCACTTCTCCCCTGG | LA-qPCR |
| R17 | POLB (mouse) | CGTGATGCCGCCGTTGAGGGTCTCCTG | LA-qPCR |
| F18 | POLB (mouse) | TATGGACCCCCATGAGGAACA | SA-PCR |
| R18 | POLB (mouse) | AACCGTCGGCTAAAGACGTG | SA-PCR |
| F19 | TUBB (mouse) | GGTACAGGGGATGTGGTTGG | LA-qPCR |
| R19 | TUBB (mouse) | GAGTCTCCTGCCTGTCCCTA | LA-qPCR |
| F20 | TUBB (mouse) | AGCTCTTCACCAGGGTCTCT | SA-PCR |
| R20 | TUBB (mouse) | TCTTTGTCTTCTGCCTCCAC | SA-PCR |
| F21 | NeuroD (mouse) | CTCGCAGGTGCAATATGAATC | LA-qPCR |
| R21 | NeuroD (mouse) | GCAACTGCATGGGAGTTTTCT | LA-qPCR |
| F22 | NeuroD (mouse) | CTGCAAAGGTTTGTCCCAGC | SA-PCR |
| R22 | NeuroD (mouse) | CTGGTGCAGTCAGTTAGGGG | SA-PCR |
| F23 | MyH4 (mouse) | GACGTGGAACTGTTAGGCCA | LA-qPCR |
| R23 | MyH4 (mouse) | AAGCCAGAGTCTTCAACCCG | LA-qPCR |
| F24 | MyH4 (mouse) | GACGACCTTGAGCTGACACT | SA-PCR |
| R24 | MyH4 (mouse) | TTGACTTTGTCCTCCTCTGC | SA-PCR |
| F25 | MyH6 (mouse) | GACAAGGGGCATTGTAGCCT | LA-qPCR |
| R25 | MyH6 (mouse) | TCTGCCTACCTTATGGGGCT | LA-qPCR |
| F26 | MyH6 (mouse) | CCAGTGGAGGACCAAGTATG | SA-PCR |
| R26 | MyH6 (mouse) | GTTCCTCTGCTTCTTGTCCA | SA-PCR |
| F27 | MyoD (mouse) | ATAGACTTGACAGGCCCCGA | LA-qPCR |
| R27 | MyoD (mouse) | GGACCGTTTCACCTGCATTG | LA-qPCR |
| F28 | MyoD (mouse) | GCGGTTCAGGACCACTTATT | SA-PCR |
| R28 | MyoD (mouse) | CCCGCTTGAGGAATAAACAT | SA-PCR |
| F29 | GAPDH (human) | CATCACTGCCACCCAGAAGA | Internal control for gene expression |
| R29 | GAPDH (human) | TTCTAGACGGCAGGTCAGGT | Internal control for gene expression |
| F 30 | 3’-UTR-PNKP (human) | GAGATCCCGTTCCGGCTATG | Expression of PNKP-3’-UTR |
| R30 | 3’-UTR PNKP (human) | CAGCGTTTATTGTGGAGGGG | Expression of PNKP-3’-UTR |
| F31 | PNKP (human) | AATACGACTCACTATAGGGAG | Sequencing for confirmation of PNKP mutation(s) in pcDNA3 |
| TV07 RPmix | NanoG (human) | RealTimePrimers.com | ChIP-qPCR |
| TV10 RPmix | Oct3/4 (human) | RealTimePrimers.com | ChIP-qPCR |
| TV11 RPmix | MyH2 (human) | RealTimePrimers.com | ChIP-qPCR |
| TV20 RPmix | β-Actin (human) | RealTimePrimers.com | ChIP-qPCR |
| HPRT1-In2-For | HPRT (human) | TGCCAGTATGGGTGGGAGAA | ChIP-qPCR |
| HPRT1ln2-Rev | HPRT (human) | AGGGTAAAAACCCAGGCATGA | ChIP-qPCR |