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[Preprint]. 2023 Aug 15:2023.08.15.553395. [Version 1] doi: 10.1101/2023.08.15.553395

Figure 5. Ectopic expression of Netrin4 by electroporation visibly affects the basement membrane underlying the coelomic epithelium, but not that underlying the endoderm, or the midline.

Figure 5.

(A, C) Electroporation of the left (A) or right (C) DM with the control, pCAG-GFP, had no effect on the basement membrane of the coelomic epithelium (arrows). Left n = 6. Right n = 4. (B, D) Electroporation of mouse Netrin4 (pCAGEN-Ntn4) and pCAG-GFP into the left (B) or right (D) DM disrupted the basement membrane underlying the coelomic epithelium (arrows) and scattered basement membrane in the mesenchyme (asterisks). Left n = 5. Right n = 5. (E) Double DM electroporations also disrupt the coelomic epithelium (arrows) but the midline appears intact despite being contacted by Ntn4+ cells (n = 3). (F, G) Electroporation of the endoderm directly with pCAG-GFP or pCAGEN-Ntn4 does not visibly affect the basement membrane underlying the endoderm (open round pointers). Control n = 5. Ntn4 n = 8. The midline appears unaffected by any of these perturbations. Scale bars = 100 um.