Table 1.
Reagents used in these experiments.
| Reagent | Source | Catalog # | Sequence | Dilution |
|---|---|---|---|---|
| Anti-laminin alpha 1 (1° ab) | Sigma | L9393 | 1:100 | |
| Anti-laminin 1 (1° ab) | DSHB | 3H11 | 1:10 | |
| Anti-perlecan (1° ab) | DSHB | 5C9 | 1:10 | |
| Anti-nidogen (1° ab) | DSHB | 1G12 | 1:10 | |
| Anti-fibronectin (1° ab) | DSHB | VA1(3) | 1:5 | |
| Anti-fibronectin (1° ab) | DSHB | B3/D6 | 1:30 | |
| Anti-fibronectin (1° ab) | Sigma | F3648 | 1:400 | |
| Anti-GM130 (1° ab) | BD Biosciences | 610822 | 1:250 | |
| Alexa Fluor 568 goat anti-rabbit (2° ab) | lnvitrogen | A-11031 | 1:500 | |
| Alexa Fluor 647 donkey anti-rabbit (2° ab) | lnvitrogen | A32795 | 1:500 | |
| Alexa Fluor 488 goat anti-mouse (2° ab) | lnvitrogen | A32723 | 1:500 | |
| DAPI | Thermofisher | D1306 | 1:2000 | |
| Dextran, Fluorescein, 3000 MW, lysine fixable, anionic | Thermofisher | D3306 | ||
| CM-Dil | lnvitrogen | C7000 | ||
| SP-DiO | lnvitrogen | D7778 | ||
| Plasmid for chordin riboprobe (chicken) | Cliff Tabin lab | T691 | ||
| Plasmid for lefty1 riboprobe (chicken) | Cepko/Tabin lab | T607 | ||
| F primer for chicken LAMA1 riboprobe from cDNA | ACGGAGAGTTTGGCAGATGA | |||
| R primer for chicken LAMA1 riboprobe from cDNA | ATCCTGAGCCCAAATCCCAA | |||
| PCR cloning kit (pDrive plasmid) | Qiagen | 231124 | ||
| pCAGEN | Connie Cepko | Addgene 11160 | ||
| pCAG-GFP | Connie Cepko | Addgene 11150 | ||
| pCI-H2B-RFP | Addgene | 92398 | ||
| Ntn4-AP-His plasmid | Addgene | 71980 | ||
| 5’ primer for cloning Ntn4 coding region out of Addgene 71980 and into pCAGEN (Xhol and Notl) | ATGCCTCGAGATATCgccaccatggggagctg | |||
| 3’ primer for cloning Ntn4 coding region out of Addgene 71980 and into pCAGEN (Xhol and Notl) | CTAGCGGCCGCGGATCCATCGATTATTACACGCAGTCTCTTTTTAAGATGTGCA |