Figure 5. rhIL16 treatment is sufficient to transiently upregulate an L1 family gene set.

(A) Scheme for experimentally validating the role of rhIL16 in L1 regulation. GSEA analysis for top, shared, concomitantly regulated (B) GO Biological Process and (C) Reactome pathway gene sets following IL16 overexpression and rhIL16 exposure for 24 hours. Shared gene sets were ranked by combining p-values from each individual treatment analysis using Fisher’s method. (D) GSEA analysis for top, differentially regulated TE family gene sets following rhIL16 exposure for 24 hours. The GSEA plot for the L1 family gene set result summarized in the bubble plot is also shown. For this plot, the FDR value is listed. In each bubble plot, the size of the dot represents the −log₁₀(FDR) and the color reflects the normalized enrichment score. FDR: False Discovery Rate. Some panels were created with BioRender.com.