Fig. 3 |. MRS2 currents.

a, Mg²⁺ transport by TmCorA or MRS2. The western blot image compares the expression of TmCorA, MRS2_FL, and MRS_EM, all tagged with a C-terminal 1D4 sequence and detected with an anti-1D4 antibody. b, MRS2 Mg²⁺ currents induced by the R332S mutation. MRS2_RS exhibits ~60% expression level of MRS2_WT. c, Inhibition of MRS2 activity by cobalt hexammine. Na⁺ currents were presented here, but similar inhibition was obtained when recording Mg²⁺ currents. d, Dose response of cobalt hexammine inhibition of MRS2 Na⁺ currents. Data points were fit with a standard saturation binding equation (black curve). Maximal inhibition achieved is ~50%, comparable with that obtained with TmCorA.