Table 2.
Antisera.
| Antigen | Immunogen Description | Source, host species, RRID | Concentration |
|---|---|---|---|
| CGRP | Synthetic CGRP peptide. | Abcam, goat polyclonal, cat# ab36001, lot #: GR3330475-13, RRID: AB_725807 | 1:1,000 |
| FoxP2 | E. coli-derived recombinant human FoxP2 isoform 1 Ala640-Glu715 | R&D Systems, sheep polyclonal cat# AF5647; lot#: CCUB0109061, RRID: AB_2107133 | 1:3,000 |
| mCherry | Full length mCherry protein | Invitrogen, rat monoclonal, cat# M11217, lot#: VA 293192, lot# W6337875, lot# VH 307508, RRID: AB_2536611 | 1:2,000 |
| GRP | Synthetic (human) bombesin coupled to bovine thyroglobulin with glutaraldehyde. | Immunostar, rabbit polyclonal, cat# 20073, lot# 1420001, RRID: AB_572221 | 1:4,000 |
| AgRP | Human AgRP 83-132 Amide | Phoenix Pharmaceuticals, rabbit polyclonal, cat#: H-003-53, lot#: 01825-2, RRID AB_2313908 | 1:5,000 |
| TH | Native tyrosine hydroxylase from rat pheochromocytoma in sheep host | Millipore, sheep polyclonal, cat#: Ab1542, lot#: 34756625, RRID: AB_90755 | 1:1,000 |
| DsRed | DsRed-express, a variant of Discosoma sp. red fluorescent protein | Takara Bio, rabbit polyclonal, cat#: 632496, lot# 1805060, RRID: AB_10013483 | 1:2,000 |
| GFP | Full length green fluorescent protein from the jellyfish Aequorea victoria | Invitrogen, chicken polyclonal, cat# A10262, lot# 2591656, lot# 1972783, RRID: AB_2534023 | 1:3,000 |
| TH | Denatured tyrosine hydroxylase from rat pheochromocytoma in rabbit host | Millipore, rabbit polyclonal, AB152, 3199177, RRID: AB_390204 | 1:10,000 |
| NPS | Synthetic NPS peptide | Abcam, rabbit polyclonal, #ab18252, lot: GR198504-1, RRID: AB_776718 | 1:2000 |