TABLE 1.

Sequences of primers used for detection of different genes by the PCR assay

Gene	Primer sequence	Ampli- con size (bp)	Refer- ence
ctxA	CTC AGA CGG GAT TTG TTA GGC ACG	301	16
	TCT ATC TCT GTA GCC CCT ATT ACG
zot	CAC TGT TGG TGA GCG TTA TCG	243	6
	CAA GCG CTG TGG GTA GAA GTG AAA
ace	GCT TAT GAT GGA CAC CCT TTA	284	6
	TTT GCC CTG CGA GCG TTA AAC
tcpA (El Tor)	GAA GAA GTT TGT AAA AGA AGA ACA C	471	16
	GAA AGG ACC TTC TTT CAC GTT G
tcpA (classical)	CAC GAT AAG AAA ACC GGT CAA GAG	617	16
	ACC AAA TGC AAC GCC GAA TGG AG
LTc	GCG ACA AAT TAT ACC GTG CT	708	33
	CCG AAT TCT GTT ATA TAT GT
STc	ATT TTT C/ATT TCT GTA TTA/G TC	105	30
	GC/TA CAG/A GCA GGA TTA CAA AA
VTc	GAA CGA AAT AAT TTA TAT GTG	521	32
	TGA TGA TGG CAA TTG AGT AT