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[Preprint]. 2023 Nov 10:2023.08.18.553709. Originally published 2023 Aug 19. [Version 2] doi: 10.1101/2023.08.18.553709

PMC10462135.1; 2023 Aug 19
PMC10462135.2; 2023 Nov 10

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Figure 6. — unc-31 null worms exhibit decreased EPSCs that are additively rescued by P334A unc-18 and open syntaxin

(A) Sample traces of spontaneous miniature postsynaptic currents (mPSCs) of wild-type (black), unc-31(e928), red, unc-31;unc-64(LE), blue, unc-31;unc-18(PA),green and the resulting triple mutant (cyan). Wild-type data is from figure 4 for purpose of representation.

(B) Amplitude of mPSCs was unchanged in all strains (one-way ANOVA, F_{(3, 40)} = 2.003; p = 0.1291). Wild-type data is from figure 4 for purpose of representation and excluded from analysis.

(B) Frequency of mPSCs. Only unc-31;unc-64(LE) worms saw an increase in mPSC frequency (one-way ANOVA, F_{(3, 40)} = 3.933; p = 0.0150), while a trend of increase was observed in the unc-31;unc-18(PA) and unc-31;unc-64(LE);unc-18(PA) mutant. Wild-type data is from figure 4 for purpose of representation and excluded from analysis.

(D) Sample traces of evoked excitatory postsynaptic currents (EPSCs) for the indicated strains. Wild-type data is from figure 4 for purpose of representation.

(E) Evoked EPSC amplitude was reduced in the unc-31 null animal when compared to control worms. A slight increase in EPSC amplitude was observed in the unc-31 ;unc-64(LE) and the unc-31;unc-64(LE);unc-18(PA) triple mutant but values did not reach statistical significance (one-way ANOVA, F_{(3, 38)} = 2.018; p = 0.1277). Wild-type data is from figure 4 for purpose of representation and excluded from analysis.

(F) EPSC amplitude when calibrated with membrane capacitance to account for differences in animal size saw the same trend (one-way ANOVA, F_{(3, 38)} = 2.949; p = 0.0449). Wild-type data is from figure 4 for purpose of representation and excluded from analysis.

(G) Charge transfer of EPSCs show a decrease in charge transfer of unc-31 null worms when compared to wild-type worms. This decrease was rescued by the unc-31;unc-64(LE) and unc-31;unc-18(PA) double mutants, and further rescued by the unc-31;unc-64(LE);unc-18(PA) triple mutant (one-way ANOVA, F_{(3, 38)} = 5.178; p = 0.0042). Wild-type data is from figure 4 for purpose of representation and excluded from analysis.

(H) Charge transfer of EPSCs calibrated to membrane capacitance to account for differences in animal size. Only unc-31;unc-64(LE);unc-18(PA) shows a significant rescue in EPSC charge transfer (one-way ANOVA, F_{(3, 38)} = 6.010; p = 0.0019). Wild-type data is from figure 4 for purpose of representation and excluded from analysis.