TABLE 2.
Inhibitory effect of calcium phosphate precipitate on gap LCR amplification and removal of inhibition by potassium acetate buffer, pH 4.1
| Centrifugation buffer | Mean (n = 2) S/CO ratio with:
|
||
|---|---|---|---|
| Water | 5 mM CaCl2 | 10 mM CaCl2 | |
| TE, pH 7.8 | 3.33 | 0.05 | 0.08 |
| 0.5 M potassium acetate, pH 4.1 | 3.13 | 2.33 | 0.03 |
| 1.0 M potassium acetate, pH 4.1 | 2.81 | 2.78 | 2.19 |