Table 2.
Imprecision of the Altona HHV-6 qPCR at low levels of HHV-6 DNA in Cerebral Spinal Fluid.
| Nominal Concentration (IU/mL) | Nominal Concentration (log10 IU/mL)) | Observed Mean (log10 copies/mL) | SD Within-Run | % CV Within-Run | SD Between-Run | % CV Between-Run | SD Total | % CV Total |
|---|---|---|---|---|---|---|---|---|
| 1000 | 3.00 | 2.77 | 0.08 | 2.77 | 0.12 | 4.41 | 0.14 | 5.12 |
| 750 | 2.88 | 2.60 | 0.09 | 3.48 | 0.12 | 4.51 | 0.14 | 5.56 |
| 500 | 2.70 | 2.32 | 0.11 | 4.60 | 0.13 | 5.64 | 0.16 | 7.10 |
| 250 | 2.40 | 2.00 | 0.18 | 9.01 | 0.31 | 15.62 | 0.35 | 17.75 |
| 125 | 2.10 | 1.55 | 0.27 | 17.48 | 0.24 | 15.35 | 0.35 | 22.43 |
IU, International Unit; SD, standard deviation; CV, coefficient of variation.