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. 2023 Aug 28;14:5249. doi: 10.1038/s41467-023-40918-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — WT C57Bl/6 and IGHV1-2 HC2 mice were sequentially immunized with NP-ovalbumin (4x) at weeks: 0, 3, 6 and 9 and ratio of NP2 to N23 binding by the serum IgG response was measured two weeks after each immunization [mean ± SD], n = 5 biologically independent animals per genotype, two-factor ANOVA. Factor 1= genotype; Factor 2 = immunization number. Following identification of an immunization number effect (P < 0.0001) but not genotype effect (P = 0.8238), factor 2 was further analyzed with Tukey’s test. The P values for the resultant pairwise comparisons are presented in the figure. Data are from one vaccination experiment.