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. 2023 Apr 13;44(9):1920–1931. doi: 10.1038/s41401-023-01083-w

Fig. 2. USP10 prevents CCND3 from K48-linked polyubiquitination.

Fig. 2

a USP10 and CCND3 plasmids were co-transfected into HEK293T cells for 48 h, followed by IP/IB assays as indicated. b USP10 and CCND3 plasmids were co-transfected with or without siUSP10 into HEK293T cells for 48 h, followed by IP/IB assays as indicated. c Purified CCND3 proteins were co-incubated with or without purified USP10 protein in a tube for 2 h, followed by IB assay, as stated in the Methods section (In-tube ubiquitination assay). d OPM2 and KMS11 cells were infected with lentiviral USP10 or empty virus (EV) for 72 h, followed by IP/IB assays as indicated. e USP10 was knocked down from OPM2 and KMS11 cells for 72 h, followed by IP/IB assays as indicated. f OPM2 and KMS11 cells were infected with lentiviral USP10 or empty virus (EV) for 72 h, followed by IP/IB assays with specific antibodies as indicated. All the experiments were performed three times independently.