Fig. 4. The N-terminal aa. 1–205 fragment is dispensable for USP10 to bind to and deubiquitinate CCND3.

a The schematic illustration of the USP10 protein. b Each truncate of USP10 was co-transfected with a CCND3 plasmid in HEK293T cells for 24 h, followed by cell lysate preparation and IP/IB assays with specific antibodies as indicated. c The truncates of USP10 were co-transfected with a CCND3 plasmid and a Ub plasmid for 24 h, followed by the measurement of CCND3 polyubiquitination with the IP/IB assays. d HEK293T cells were co-transfected with HA-Ub, Flag-CCND3 or Flag-PTEN with WT or C424 USP10 plasmids for 24 h, followed by IP/IB assays as indicated.