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. 2023 May 4;44(9):1867–1878. doi: 10.1038/s41401-023-01094-7

Fig. 1. Bergapten (BeG) attenuates nucleotide-binding domain and leucine-rich repeat-containing (NLR) family pyrin domain-containing protein 3 (NLRP3) inflammasome activation.

Fig. 1

a Molecular structure of BeG. b Cytotoxicity of J774A.1 cells after 12 and 24 h of BeG treatment (n = 3 per group). c–e Western blotting analysis of NLRP3, pro-caspase1, apoptosis-associated speck-like protein containing a CARD (ASC) and NLRP3 inflammasome activation-related protein (Casp1-p10) from the cell (J774A.1 and bone marrow-derived macrophages [BMDMs]) lysates and culture supernatants. f–h Effect of BeG treatment on interleukin-1β (IL-1β) production in the culture supernatants of BMDMs (n = 3 per group). i–l Images of immunofluorescence showing the subcellular distribution of ASC (green). Nuclei (blue) are shown by Hoechst 33342 (arrowheads mark ASC specks); scale bar: 20 μm. Quantification of ASC specks was done from six random fields (one field per well) (n = 6 per group). Data shown as mean ± SD (****P < 0.0001; ns not significant).