Table 1.
Gene | Forward primer | Reverse primer |
---|---|---|
IL1B | 5′-TTCGACACATGGGATAACGAGG-3′ | 5′-TTTTTGCTGTGAGTCCCGGAG-3′ |
IL6 | 5′-ACTCACCTCTTCAGAACGAATTG-3′ | 5′-CCATCTTTGGAAGGTTCAGGTTG-3′ |
Il1b | 5′-CTGTGACTCATGGGATGATGATG-3' | 5′-CGGAGCCTGTAGTGCAGTTG-3' |
Il6 | 5′-CTGCAAGAGACTTCCATCCAG-3′ | 5′-AGTGGTATAGACAGGTCTGTTGG-3′ |
Tnf | 5′-CCTGTAGCCCACGTCGTAG-3′ | 5′-GGGAGTAGACAAGGTACAACCC-3′ |
Tgfb1 | 5′-CTCCCGTGGCTTCTAGTGC-3′ | 5′-GCCTTAGTTTGGACAGGATCTG-3′ |
D-loop | 5′-AATCTACCATCCTCCGTGAAACC-3′ | 5′-TCAGTTTAGCTACCCCCAAGTTTAA-3′ |
Tert | 5′-CTAGCTCATGTGTCAAGACCCTCTT-3' | 5′-GCCAGCACGTTTCTCTCGTT-3' |
Relative gene expression levels were analyzed by relative quantitative 2−ΔΔCT method.