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. 2023 May 4;44(9):1867–1878. doi: 10.1038/s41401-023-01094-7

Table 1.

Primer sequence (forward and reverse).

Gene Forward primer Reverse primer
IL1B 5′-TTCGACACATGGGATAACGAGG-3′ 5′-TTTTTGCTGTGAGTCCCGGAG-3′
IL6 5′-ACTCACCTCTTCAGAACGAATTG-3′ 5′-CCATCTTTGGAAGGTTCAGGTTG-3′
Il1b 5′-CTGTGACTCATGGGATGATGATG-3' 5′-CGGAGCCTGTAGTGCAGTTG-3'
Il6 5′-CTGCAAGAGACTTCCATCCAG-3′ 5′-AGTGGTATAGACAGGTCTGTTGG-3′
Tnf 5′-CCTGTAGCCCACGTCGTAG-3′ 5′-GGGAGTAGACAAGGTACAACCC-3′
Tgfb1 5′-CTCCCGTGGCTTCTAGTGC-3′ 5′-GCCTTAGTTTGGACAGGATCTG-3′
D-loop 5′-AATCTACCATCCTCCGTGAAACC-3′ 5′-TCAGTTTAGCTACCCCCAAGTTTAA-3′
Tert 5′-CTAGCTCATGTGTCAAGACCCTCTT-3' 5′-GCCAGCACGTTTCTCTCGTT-3'

Relative gene expression levels were analyzed by relative quantitative 2−ΔΔCT method.