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. 2023 Aug 28;14:5237. doi: 10.1038/s41467-023-40900-y

Fig. 4. On-phage two-step enzymatic extension of bi-antennary N-glycan by B4GalT1 and Pd26ST.

Fig. 4

a Sequence of β-(1→4)-galactosylation and α-(2→6)-linked sialylation to afford homogeneous sialylated product P2. b MALDI-TOF MS: (b) substrates S1, S2 were products of the ligation reaction. (as described in Fig. 2) (c) B4GalT1-catalyzed galactosylation converted S1 to P1. d Pd26ST-catalyzed sialylation converted P1 to P2 yielding a homogeneous display of P2. c Conditions for enzymatic reactions. d Detailed changes of each peak on MALDI-TOF MS. The lightning symbol indicate that these peaks are “ghost” peaks that do not respond to β-galactosidase treatment.