Figure 3.

Deletion of wciE results in loss of branching αGalp from 33F PS.A, recombinant strategy using Sweet Janus cassette to create the PATH3618 wciE knock-out mutant, FG14. B, ¹H NMR spectra of native capsule PS purified from PATH3618, FG14, and 212862. Signals arising from each monosaccharide residue are assigned as labeled in Figure 2, C and D, as well as in Table 1. Residue Eꞌ represents the de-O-acetylated form of glycosyl residue E. NMR, nuclear magnetic resonance; PS, polysaccharide.