Table 1.
1H and13C chemical shifts (ppm) of serotypes 33F and 33E obtained at 35 °C
| Residue | Label | H1/C1 | H2/C2 | H3/C3 | H4/C4 | H5/C5 | H6a,H6b/C6 | O-Ac |
|---|---|---|---|---|---|---|---|---|
| Serotype 33F | ||||||||
| →3-β-D-Galp-(1→ | A | 4.69/105.8 | 3.70/72.1 | 3.70/83.1 | 4.10/70.6 | 3.73/76.8 | 3.76,3.78/63.0 | |
| →2,3-α-D-Galp-(1→ | B | 5.40/98.8 | 4.20/70.86 | 4.20/78.3 | 4.29/71.4 | 4.17/73.0 | 3.80,3.85/63.0 | |
| →3-β-D-Galf-(1→ | C | 5.36/110.5 | 4.42/81.5 | 4.15/87.2 | 4.33/83.9 | 4.00/72.1 | 3.70,3.71/65.3 | |
| →3-β-D-Glcp-(1→ | D | 4.67/103.9 | 3.49/75.5 | 3.68/84.3 | 3.50/70.2 | 3.51/77.8 | 3.94,3.95/63.0 | |
| →5-β-D-Galf-(1→ | E | 5.39/109.0 | 5.07/86.0 | 4.51/77.0 | 4.30/84.7 | 4.13/79.3 | 3.74,3.75/63.1 | 2.17/22.5 |
| Eꞌ | 5.21/111.1 | 4.22/83.4 | 4.30/77.8 | 4.19/83.5 | 4.10/79.1 | 3.74,3.75/63.1 | ||
| →α-D-Galp-(1→ | F | 5.14/96.8 | 3.86/70.0 | 3.95/71.3 | 3.98/71.7 | 4.35/72.9 | 3.78,3.79/63.0 | |
| Serotype 33E | ||||||||
| →3-β-D-Galp-(1→ | A | 4.68/106.1 | 3.76/72.5 | 3.78/81.9 | 4.10/70.6 | 3.73/76.8 | 3.76,3.78/63.0 | |
| →3-α-D-Galp-(1→ | B | 5.11/101.6 | 4.05/69.3 | 4.05/81.0 | 4.29/71.4 | 4.14/73.2 | 3.80,3.85/63.0 | |
| →3-β-D-Galf-(1→ | C | 5.36/110.4 | 4.40/81.7 | 4.11/86.7 | 4.34/84.1 | 3.91/72.9 | 3.70,3.73/65.0 | |
| →3-β-D-Glcp-(1→ | D | 4.67/103.9 | 3.49/75.6 | 3.70/84.2 | 3.49/70.4 | 3.50/78.0 | 3.94,3.95/63.0 | |
| →5-β-D-Galf-(1→ | E | 5.40/109.0 | 5.06/86.0 | 4.51/77.0 | 4.30/84.7 | 4.13/79.3 | 3.74,3.75/63.1 | 2.17/22.5 |
| Eꞌ | na | 4.22/83.4 | 4.30/77.8 | 4.19/83.5 | 4.10/79.1 | 3.74,3.75/63.1 | ||
Each carbohydrate residue is labeled with a unique letter for 33F (A-F) and 33E (A–E). Residue Eꞌ represents the de-O-acetylated form of glycosyl residue E. Proton and carbon atoms are indicated by letters H and C, respectively, and the numbers associated with them indicate their respective position. A slash separates the proton and carbon chemical shifts (ppm). For each residue, the table shows the chemical shifts of every proton and carbon molecule attached to it at different positions.
Abbreviations: na, not assigned due to overlap with impurity and/or weak intensity; O-Ac, O-acetylation.