Table 2.
Strains phenotypically characterized in this study
| Strain | cps genotype | Reference factor sera reactivity |
FCSAa |
NMRb | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Pool T | Pool E | 33b | 33e | 33f | 6a | 20b | Profilec | 33FM1d | 33FG1 | |||
| STREP33F | 33F | [+] | [+] | [+] | -- | -- | -- | -- | 33F | [+] | [+] | 33F |
| 212862 | 33F-1c | [+] | [+] | [+] | -- | -- | -- | -- | 33F | -- | [+] | 33E |
| PATH3618 | 33F-1a | [+] | [+] | [+] | -- | -- | -- | -- | 33F | [+] | [+] | 33F |
| FG14 | 33F-1a, wciE::SJCe | [+] | [+] | [+] | -- | -- | -- | -- | 33F | -- | [+] | 33E |
| Reference strains | ||||||||||||
| SSISP33F/2 | naf | [+] | [+] | [+] | -- | -- | -- | -- | 33F | [+] | [+] | na |
| SSISP33A/2 | na | [+] | [+] | [+] | -- | -- | -- | [+] | 33A | na | na | na |
| SSISP33B/2 | na | [+] | [+] | -- | -- | [+] | -- | -- | 33B | na | na | na |
| SSISP33C/2 | na | [+] | [+] | -- | [+] | [+] | -- | -- | 33C | -- | -- | na |
| SSISP33D/2 | na | [+] | [+] | -- | -- | [+] | [+] | -- | 33D | na | na | na |
a
FCSA, flow cytometric serotyping assay using anti-33F monoclonal antibodies.
b
Capsule PS structure as determined by nuclear magnetic resonance (NMR) analysis in this study.
c
profile according to conventional serotyping criteria.
d
The 33FM1 cell line is unfortunately lost. However, our laboratory has archived the hybridoma supernatant for future experiments.
e
Recombination deletion of wciE gene with Sweet Janus Casette (SJC).
f
na, not applicable/not determined; [+], positive; --, negative.