Table 1.

Experimental kinetic parameters for ScNep1, its three mutated variants (all measured at 30 ^∘C), and AfNep1 (measured at 55 ^∘C).

	k${}_{cat}$ (s−1)	KM (mM)	Relative k${}_{cat}$
ScNep1
WT	0.88 ± 0.05	16.3 ± 1.90	1.0
D101A	0.050 ± 0.001	1.40 ± 0.16	0.06
S233A	0.61 ± 0.04	13.3 ± 2.10	0.69
S233M	0.23 ± 0.02	7.03 ± 1.20	0.26
AfNep1
WT	0.20 ± 0.01	2.82 ± 0.20	–