Table SIII.
Results-cost description per NSCLC sample and patient (€ 2019).
| Item | Professional | Disposable | Technology | Total cost per | ||||
|---|---|---|---|---|---|---|---|---|
| Time (hour) (A) | Cost (hour) (B) | Volume (C) | Cost (unit) (D) | Volume (E) | Cost (year) (F) | Samplea | Patientb | |
| Step 2 - Analysis - Staining | ||||||||
| Substep 2a - H&E staining | ||||||||
| Laboratory technician | 0.05 | € 27.28 | - | - | - | - | € 0.66 c | € 1.14 |
| H&E stainers | - | - | - | - | 144,586 d | € 36,238.50 | € 1.16 e | € 1.99 |
| Slides | - | - | 4.63 | € 0.04 | - | - | € 0.19 | € 0.33 |
| H&E reagent | - | - | 4.63 | € 0.21 | - | - | € 0.97 | € 1.67 |
| Total Substep 2a - Professional | € 0.66 | € 1.14 | ||||||
| Total Substep 2a - Disposable | € 1.16 | € 1.99 | ||||||
| Total Substep 2a - Technology | € 1.16 | € 1.99 | ||||||
| Overall Substep 2a | € 2.97 | € 5.11 | ||||||
| Substep 2b - IHC, FISH and Special stainings | ||||||||
| Graduated technician (FISH staining only) | 0.75 | € 35.07 | - | - | - | - | € 5.63 f | € 9.68 |
| Laboratory technician (FISH staining only) | 0.40 | € 27.28 | - | - | - | - | € 2.34 f | € 4.02 |
| Laboratory technician (IHC and Special stainings) | 0.05 | € 27.28 | - | - | - | - | € 3.34 f | € 5.74 |
| IHC stainers | - | - | - | - | 66,732 g | € 163,625 | € 5.99 h | € 10.30 |
| IHC staining reagent | - | - | 1 | € 8.00 | - | - | € 7.96 i | € 13.69 |
| Predictive IHC staining reagent | - | - | 1 | € 50.00 | - | - | € 72.44 j | € 124.58 |
| FISH stainers | - | - | 1 | € 150.00 | - | - | € 32.11 k | € 55.22 |
| Special staining reagent + equipment | - | - | 1 | € 4.00 | - | - | € 0.01 l | € 0.02 |
| Total Substep 2b - Professional | € 11.30 | € 19.43 | ||||||
| Total Substep 2b - Disposable | € 112.52 | € 193.50 | ||||||
| Total Substep 2b - Technology | € 5.99 | € 10.30 | ||||||
| Overall Substep 2b | € 129.82 | € 223.25 | ||||||
| Substep 2c - Slides preparation | ||||||||
| Laboratory technician | 0.08 | € 27.28 | - | - | - | - | € 16.07 h | € 27.64 |
| Total Substep 2c - Professional | € 16.07 | € 27.64 | ||||||
| Overall Substep 2c | € 16.07 | € 27.64 | ||||||
| Total Step 2 - Professional | € 28.03 | € 48.20 | ||||||
| Total Step 2 - Disposable | € 113.68 | € 195.50 | ||||||
| Total Step 2 - Technology | € 7.15 | € 12.30 | ||||||
| Overall Step 2 | € 148.87 | € 256.01 | ||||||
a Cost per sample=[(A*B)+(C*D)+(F/E)] unless otherwise stated.
b Cost per patient=[Cost per sample*(724/421)], where 724=number of NSCLC tissue samples and 421=number of NSCLC patients.
c Cost per sample=[(A*B)*(3351/724)], where 3351= number of NSCLC tissue slides undergoing H&E staining and 724=number of NSCLC tissue samples.
d Assuming that [(65%*222,440 slides)=144,586 slides undergo H&E staining.
e Cost per sample=[(F/E)*4.63], where 4.63=average number of slides per NSCLC H&E staining.
f Cost per sample=[(A*B)*(155/724)], where 155= number of FISH performed on NSCLC tissue slides and 724=number of NSCLS tissue samples.
g Assuming that (35%*222,440 slides)=66,732 slides undergo IHC, FISH and/or Special stainings.
h Cost per sample={(F/E)*[(720+1049)/724]}, where 720=number of NSCLC IHC slides, 1049=number of NSCLC predictive IHC slides and 724=number of NSCLC tissue samples.
i Cost per sample=[(C*D)*(1049/724)], where 1049=number of NSCLC predictive IHC slides and 724=number of NSCLC tissue samples.
j Cost per sample=[(C*D)*(720/724)], where 720=number of NSCLC IHC slides and 724=number of NSCLC tissue samples.
k Cost per sample=[(C*D)*(155/724)], where 155= number of FISH performed on NSCLC tissue slides and 724=number of NSCLS tissue samples.
l Cost per sample=[(C*D)*(2/724)], where 2= number of Special stainings performed on NSCLC tissue slides and 724=number of NSCLS tissue samples.
m Cost per sample=[(A*B)*(5120/724)], where 5120=number of NSCLC tissue slides and 724=number of NSCLC tissue samples.