TABLE 1.

Oligonucleotide sequences for XL and nested PCR amplification of HIV-2 proviral DNA

Primer	Position	Orientation	Nucleotide sequence	PCR type	Source or reference
PFD1	4729–4749	Sense	5′-GGTCTATTWCAGAGAAGGCAG-3′	XL	In house
LTR9574	9574–9592	Antisense	5′-TGGTGAGAGTCTAGCAGGG-3′	XL	Berry et al. (2)
LTR1	75–96	Sense	5′-TTCCCTGCTRGACTCTCACCAG-3′	XL	In house
LTR2	8935–8953	Antisense	5′-ACATCCCTTCCAGTCCCCC-3′	XL	In house
EB0	6300–6324	Sense	5′-ATACAGTGCTTRCCAGACAATGATG-3′	Nested	In house
EB6	6945–6963	Antisense	5′-CCATTRAAGCCAAACCAWGT-3′	Nested	In house
EB2	6783–6804	Sense	5′-TCATGTGAYAARCAYTATTGGG-3′	Nested	In house
EB5	7310–7329	Antisense	5′-CTCCTCTGCAGTTAGTCCAC-3′	Nested	In house
EB7	7149–7165	Sense	5′-CCYAGGCAAGCATGGTG-3′	Nested	In house
ENVE	7920–7939	Antisense	5′-GCACATCCCCATGAATTTAG-3′	Nested	In house
ENVA	7691–7723	Sense	5′-GCTAGGGTTCTTGGGTTTTCTCGC(AG)ACAGCAGG-3′	Nested	Gao et al. (6)
ENVB	8415–8425	Antisense	5′-CAAGAGGCGTATCAGCTGGCGGATCAGGAA-3′	Nested	Gao et al. (6)
ENVF	8265–8291	Sense	5′-TATAGGCCWGTTTTCTCTTCCCCYCCC-3′	Nested	In house
ENVG	8557–8580	Antisense	5′-CTTCTTGGATCCACTCGCACCCAT-3′	Nested	In house