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. 2023 Jul 13;299(9):105047. doi: 10.1016/j.jbc.2023.105047

Figure 6.

Figure 6

Decreasing PDXP activity restores cellular PLP content in PLPHP-deficient HEK293 cells.A, the effect of nonspecific PDXP inhibition with 80 mM sodium phosphate (NaPi) for 60 min on the content of 13C4-labeled B6 vitamers in control and PLPHP-deficient HEK293 cells. Data are means from n = 3 per cell line and condition, ±SD. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared to 0 mM NaPi at the same genotype (unpaired t test). B, the effect of PDXP knockdown with siRNA for 48 h on PDXP mRNA levels in control and PLPHP-deficient HEK293 cells. Data are means from n = 3 per cell line and condition, ±SD. ∗∗∗p < 0.001 compared to control siRNA at the same genotype (unpaired t test). C, the effect of PDXP knockdown with siRNA for 48 h on B6 vitamer content in control and PLPHP-deficient HEK293 cells. Data are means from n = 3 per cell line and condition, ±SD. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared to control siRNA at the same genotype (unpaired t test). NT, not transfected. PDXP, pyridoxal phosphatase; PLP, pyridoxal 5'-phosphate; PLPHP, pyridoxal 5′-phosphate homeostasis protein.