Table 4.
Primers used for real-time quantitative PCR analysis.
| Genes | Forward primer (5′–3′) | Reverse primer (5′–3′) | Accession number |
|---|---|---|---|
| MyoG | TTACGAAGGCGGCGATAACTT | TGGTGAGGAGACATGGACAGA | JQ793897 |
| MyoD | ATGGAGTTGTCGGATATTCCCTTC | GCGGTCAGCGTTGGTTGTT | MG544985 |
| Mrf4 | TCGCTCCTGTATTGATGTTGATGA | GCTCCTGTCTCGCATTCGTT | KT899334 |
| Myf5 | GTGCCTGTGCCTCATCTCCT | AATGCGTGGTTCACCTTCTTCA | GU290227 |
| MyHC | GACGCTCATCACCACCAACC | TGCTCCTCACGCTGCTTCT | EU414733 |
| FGF6a | CGCATACGAGTCTTCCAT | CCTACGAGAACATCCAACA | MK050993 |
| FGF6b | TCCAGTCCGCTTCCGAGTA | AGATGAAACCCGATGCCTACA | MK050992 |
| MSTN | CTGACGCCAAGTTCCACATACA | CGACTCTGCTTCAAGTTCTTCTCT | KP719016 |
| TOR | TCCCACTTTCCACCAACT | ACACCTCCACCTTCTCCA | JX854449 |
| β-Actin | TATGTTGGTGACGAGGCTCA | GCAGCTCGTTGTAGAAGGTG | M25013 |
MyoG = myogenin; MyoD = myoblast determination protein; Mrf4 = myogenic regulatory factor 4; Myf5 = myogenic factor 5; MyHC = myosin heavy chain; FGF6a = fibroblast growth factor 6a; FGF6b = fibroblast growth factor 6b; MSTN = myostatin; TOR = target of rapamycin.